A dark yellow fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh) for Forster resonance energy transfer with GFP

Ultramarine, a Chromoprotein Acceptor for Förster Resonance Energy Transfer

We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; ε(585 nm), 64,000 M(-1) x cm(-1)) for use as a Förster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring activation of caspase 3 in live cells using fluorescence lifetime imaging. Ultramarine has the potent...

Corrigendum: A dark green fluorescent protein as an acceptor for measurement of Förster resonance energy transfer

This corrects the article DOI: 10.1038/srep15334.

A dark green fluorescent protein as an acceptor for measurement of Förster resonance energy transfer

Measurement of Förster resonance energy transfer by fluorescence lifetime imaging microscopy (FLIM-FRET) is a powerful method for visualization of intracellular signaling activities such as protein-protein interactions and conformational changes of proteins. Here, we developed a dark green fluorescent protein (ShadowG) that can serve as an acceptor for FLIM-FRET. ShadowG is spectrally similar t...

Additional correction for energy transfer efficiency calculation in filter-based Forster resonance energy transfer microscopy for more accurate results.

Forster resonance energy transfer (FRET) microscopy is commonly used to monitor protein interactions with filter-based imaging systems, which require spectral bleedthrough (or cross talk) correction to accurately measure energy transfer efficiency (E). The double-label (donor+acceptor) specimen is excited with the donor wavelength, the acceptor emission provided the uncorrected FRET signal and ...

Fluorescence Resonance Energy Transfer using Color Variants of GFP